BRASILIA, Sept. 25 (Xinhua) -- Ana Paula de Oliveira, female Brazilian soccer referee actually under criticism, didn't present herself to a physical test on Tuesday and will lose her functions as qualified referee of FIFA.

Ana Paula, who just posed nude for men magazine Playboy, didn't show up for muscular problems in the complexes of Maracana, where all FIFA referee title holders should pass a physical test in the stadium of athletics Celio de Barros, in the north of Rio de Janeiro.

It was the last chance for referees and assistants to stay with FIFA's qualified referee team.

Besides Ana Paula, Wilson Luiz Seneme of Sao Paulo didn't show up for injury, becoming the other to leave the group of international referees.

They should also stop to whistle the Brazilian Championship until they pass an evaluation of the Brazilian Football Confederation (CBF), which will be organized in 40 days.